Expression and pharmacological characterization of alpha(1)-adrenoceptors in rat seminal vesicle

Expression and pharmacological characterization of alpha(1)-adrenoceptors in rat seminal vesicle

Author Silva, M. A. Google Scholar
Megale, A. Google Scholar
Avellar, MCW Google Scholar
Porto, C. S. Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract In the present study, we investigated the alpha(1)-adrenoceptor subtypes mediating adrenaline-induced contractions of the rat seminal vesicle by using functional studies. the reverse transcription combined with polymerase chain reaction (RT-PCR) was also used to identify of alpha(1)-adrenoceptor mRNA subtypes. the rank order of potency of alpha(1)-adrenoceptor antagonists in blocking the contractile effects of adrenaline was: prazosin = WB 4101 >>> BMY 7378 > chloroethylclonidine, indicating the presence of alpha(1A)-adrenoceptors in the rat seminal vesicle. in the presence of nifedipine, there was a 76% reduction in the adrenaline-induced contractions. the nifedipine-insensitive component (24%) of the contractile response to adrenaline was unaffected by chloroethylclonidine. A small pool of spare alpha(1)-adrenoceptors for adrenaline (0.10%) was also detected. All three alpha(1)-adrenoceptor subtypes were amplified when RT-PCR was performed on total RNA isolated from rat seminal vesicle. in conclusion, these data indicate the presence of three alpha(1)-adrenoceptor mRNA subtypes, but only alpha(1A)-adrenoceptors are involved in the rat seminal vesicle contraction. (C) 1999 Elsevier Science B.V. All rights reserved.
Keywords seminal vesicle
alpha(1)-adrenoceptor mRNA subtype
smooth muscle
Language English
Date 1999-09-24
Published in European Journal of Pharmacology. Amsterdam: Elsevier B.V., v. 381, n. 2-3, p. 141-149, 1999.
ISSN 0014-2999 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 141-149
Access rights Closed access
Type Article
Web of Science ID WOS:000083143000009

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