Phenotypic and genetic features of Escherichia coli strains showing simultaneous expression of localized and diffuse adherence

Phenotypic and genetic features of Escherichia coli strains showing simultaneous expression of localized and diffuse adherence

Author Scaletsky, Isabel Cristina Affonso Autor UNIFESP Google Scholar
Pedroso, Margareth Zabeu Autor UNIFESP Google Scholar
Silva, Rosa Maria Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract We have previously shown that some Escherichia coli strains isolated from children with diarrhea present the so-called 'localized and diffused adherence (LA/DA) pattern' in which both localized adherence (LA) and diffused adherence (DA) are expressed simultaneously. in the present study, we show that the LA adherence of these strains is genetically and phenotypically similar to that so far described for enteropathogenic E. coli (EPEC) as determined by DNA hybridization and electron microscopy. On the other hand, the DA is encoded by genes not homologous to the DAEC or AIDA-I DNA probes. in addition, the LA/DA strains are able to invade eukaryotic cells both in vitro and in vivo. in the rabbit ileal loop assay their invasion capacity goes beyond the enterocyte and reaches the muscularis mucosae as determined by transmission electron microscopy. These findings suggest that the LA/DA adherence pattern may be linked to a new E. coli virulence category which in the case of the strains studied may be associated to other virulence traits that enable them to more deeply invade the intestinal mucosa. (C) 1999 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
Keywords diarrhea
Escherichia coli
adhesion
localized adherence
diffuse adherence
invasion
Language English
Date 1999-03-01
Published in Fems Immunology and Medical Microbiology. Amsterdam: Elsevier B.V., v. 23, n. 3, p. 181-188, 1999.
ISSN 0928-8244 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 181-188
Origin http://dx.doi.org/10.1111/j.1574-695X.1999.tb01237.x
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000079276600001
URI http://repositorio.unifesp.br/handle/11600/26038

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