Involvement of the major glycoprotein (gp43) of Paracoccidioides brasiliensis in attachment to macrophages

Involvement of the major glycoprotein (gp43) of Paracoccidioides brasiliensis in attachment to macrophages

Author Almeida, SR Google Scholar
Unterkircher, C. S. Google Scholar
Camargo, Z. P. Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
UNESP
Abstract The yeast form of Paracoccidioides brasiliensis, the causative agent of a deep mycosis in humans, is known to be phagocytized by, and to multiply inside, macrophages. in this work we describe the involvement of gp43, a major antigenic protein of P. brasiliensis, in the initial steps of attachment of the fungus to macrophages. Anti-gp43 F(ab) polyclonal fragments were capable of inhibiting phagocytosis in a concentration-dependent manner. Sheep red blood cells sensitized with purified gp43 were more endocytized than SF:BC alone, and this process was also inhibited by anti-gp43 F(ab) fragments. Inhibition tests indicated the involvement of fucose and mannose residues in the phagocytosis of the fungus and of SRBC-gp43 by macrophages. Taken together, these results suggest that gp43 may be involved in the adherence and uptake of the fungus by murine peritoneal macrophages, and that this binding may be dependent on monosaccharide residues that are part of the gp43 glycoprotein.
Keywords macrophages
Paracoccidioides brasiliensis
paracoccidioidomycosis
phagocytosis
Language English
Date 1998-12-01
Published in Medical Mycology. Oxford: Blackwell Science Ltd, v. 36, n. 6, p. 405-411, 1998.
ISSN 1369-3786 (Sherpa/Romeo, impact factor)
Publisher Blackwell Science Ltd
Extent 405-411
Origin http://dx.doi.org/10.1080/02681219880000641
Access rights Closed access
Type Article
Web of Science ID WOS:000077858400008
URI http://repositorio.unifesp.br/handle/11600/25993

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