c-Fos expression induced by electroacupuncture at the Zusanli point in rats submitted to repeated immobilization

c-Fos expression induced by electroacupuncture at the Zusanli point in rats submitted to repeated immobilization

Author Medeiros, M.a. Google Scholar
Canteras, N.s. Google Scholar
Suchecki, Deborah Autor UNIFESP Google Scholar
Mello, Luiz Eugenio Araujo de Moraes Autor UNIFESP Google Scholar
Institution Universidade Federal Rural do Rio de Janeiro Departamento de Ciências Fisiológicas
Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Abstract In laboratory animals, acupuncture needs to be performed on either anesthetized or, if unanesthetized, restrained subjects. Both procedures up-regulate c-Fos expression in several areas of the central nervous system, representing therefore a major pitfall for the assessment of c-Fos expression induced by electroacupuncture. Thus, in order to reduce the effect of acute restraint we used a protocol of repeated restraint for the assessment of the brain areas activated by electroacupuncture in adult male Wistar rats weighing 180-230 g. Repeated immobilization protocols (6 days, 1 h/day and 13 days, 2 h/day) were used to reduce the effect of acute immobilization stress on the c-Fos expression induced by electroacupuncture at the Zusanli point (EA36S). Animals submitted to immobilization alone or to electroacupuncture (100 Hz, 2-4 V, faradic wave) in a non-point region were compared to animals submitted to electroacupuncture at EA36S (4 animals/subgroup). c-Fos expression was measured in 41 brain areas by simple counting of cells and the results are reported as number of c-Fos-immunoreactive cells/10,000 µm². The protocols of repeated immobilization significantly reduced the immobilization-induced c-Fos expression in most of the brain areas analyzed (P < 0.05). Animals of the EA36S groups had significantly higher levels of c-Fos expression in the dorsal raphe nucleus, locus coeruleus, posterior hypothalamus and central medial nucleus of the thalamus. Furthermore, the repeated immobilization protocols intensified the differences between the effects of 36S and non-point stimulation in the dorsal raphe nucleus (P < 0.05). These data suggest that high levels of stress can interact with and mask the evaluation of specific effects of acupuncture in unanesthetized animals.
Keywords Acupuncture
Zusanli point
Immediate early genes
Language English
Date 2003-12-01
Published in Brazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 36, n. 12, p. 1673-1684, 2003.
ISSN 0100-879X (Sherpa/Romeo, impact factor)
Publisher Associação Brasileira de Divulgação Científica
Extent 1673-1684
Origin http://dx.doi.org/10.1590/S0100-879X2003001200009
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000187175000009
SciELO ID S0100-879X2003001200009 (statistics in SciELO)
URI http://repositorio.unifesp.br/handle/11600/1924

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